Whole Exome and Whole Genome Profiling as well Genome-Wide Methylation Profiling from Early to Advanced Chronic Lymphocytic leukemia (CLL), Genome-Wide Methylation Profiling in Monoclonal B Cell Lymphocytosis (MBL)

Serial whole exome sequencing of samples collected from 21 patients at multiple time points during progression from early to late chronic lymphocytic leukemia (CLL) and one matching non-tumor tissue sample from each patient. Additional whole genome sequencing of samples from 3 selected patients. Genome-wide B cell methylation compared between 5 healthy donors from the same age group as patients, 20 patients with monoclonal B cell lymphocytosis and in serial CLL samples with matching whole exome sequencing. Moreover, genome-wide single-cell RRBS-profiling of B cells from one healthy donor and from one patient with CLL was performed using a protocol newly developed by the A. Meissner group.]]>
Chronic lymphocytic leukemia (CLL) patients were included into serial analysis if they had an established diagnosis of CLL and at for serial analysis least 2 serial samples available spanning a time period of at least 1 year between diagnosis and close to first treatment. Monoclonal B cell lymphocytosis (MBL) samples were included based on established diagnosis of MBL and based on previous characterization (Puente XS et al., Nature 2015; PMID: 26200345.) Healthy donors were included if they had no evidence for a hematologic disease (all) and were aged over 60 years at time of sample acquisition (for eRRBS).]]>