RNA-Sequencing to identify the transcriptional targets of Rai1

The goals of this study are to harvest Rai1-deficient mouse brains from multiple tissues and timepoints and compare the transcriptome with their wild-type littermates. Methods: mRNA profiles from Rai1 conditional knockouts and wild-type mice were generated by deep sequencing, in duplicate using Illumina system. Conclusions: Our study suggests that Rai1 preferentially regulate genes involved in cell adhesion and neural projection. Cortical, striatal, and hypothalamic mRNA profiles of wild-type and Rai1 conditional knockout mice were generated by deep sequencing, in duplicates.本研究的目标为从多种组织、多个时间点采集Rai1缺陷型小鼠的脑组织，并将其转录组与同窝野生型小鼠的转录组进行对比。 方法：采用Illumina测序系统进行双重复深层测序，获取Rai1条件性敲除小鼠与野生型小鼠的mRNA表达谱。 结论：本研究表明，Rai1可优先调控参与细胞黏附与神经投射的基因。 本研究对野生型与Rai1条件性敲除小鼠的皮层、纹状体及下丘脑组织的mRNA表达谱进行了深层测序，实验设置双重复样。