Effect of lipopolysachharide (LPS) on the expression of inflammatory mRNAs in the mouse oviduct

Our objective was to determine the effect of intraperitoneal (IP) administration of E. Coli-derived lipopolysaccharide (LPS) on the acute expression of inflammatory mRNAs in the mouse oviduct On the day of estrus, 6-8 week old CD1 mice were treated IP with 0 (control), 2 µg (low dose) or 10 µg (high dose) of LPS from E. Coli serotype 055:B5 in 100 μL PBS. Mice were killed 24 h later and the oviducts collected for determination of the effect of treatment on inflammatory mRNAs (n=4/treatment) by a targeted nanostring approach using the nCounter GX Mouse Inflammation Kit (Nanostring Technologies, Seattle, WA). Real-time PCR was then used to verify a selection of mRNAs. The effect of LPS on the expression of mRNAs was evaluated by one-way ANOVA and treatment means of differentially expressed mRNA (P<0.05) were separated using a post-hoc LSD test.