Analysis of the dose- and time-dependent mutagenic effects of benzo[b]fluoranthene in MutaMouse using novel error-corrected sequencing and conventional toxicology tools to advance genetic toxicology testing.

The overarching objective of this project is focusing on the creation of foundational data to establish Duplex Sequencing (DS), an error-corrected next-generation sequencing technology, as a regulatory assay for in vivo mutagenicity testing. DS was applied using a 48 kb panel of endogenous loci spread over the various mouse chromosomes to determine chemically induced mutagenicity. Mouse males were exposed to increasing doses and durations of benzo[b]fluoranthene, a polycyclic aromatic hydrocarbon, and we extracted the DNA of somatic tissues.