Transcription profiling by array of mouse embryos at 8 different stages

The experiment were perfomed as a part of our Vertebrate Evo-Devo project. The aim of the project is to compare transcription profiles of normal (unmanipulated, wild-type, whole embryo) vertebrate embryos. Total RNA was collected from wild type C57BL/6 mice, whole embryos at 8 different stages (Stages:E7.5, E8.5, E9.5, E10.5, E12.5, E14.5, E16.5, E18.5), and hybridized to A-AFFY-45 Mouse Genome 430 2.0 Array. All the stages contains data from 2 to 3 biological replications. Each staged-samples consists of pooled total RNA from several whole embryos.本实验系我们的脊椎动物进化发育（Vertebrate Evo-Devo）项目的组成部分。该项目的目标是比对正常（未人工干预、野生型、完整胚胎）脊椎动物胚胎的转录谱（transcription profiles）。研究人员从野生型C57BL/6小鼠的8个不同发育阶段（E7.5、E8.5、E9.5、E10.5、E12.5、E14.5、E16.5、E18.5）的完整胚胎中提取总RNA，并将其与A-AFFY-45 小鼠基因组430 2.0芯片（Mouse Genome 430 2.0 Array）进行杂交。所有发育阶段的样本均包含2至3次生物学重复的数据。每个发育阶段的样本均由多个完整胚胎的总RNA混合制备得到。