DNA Sequencing of Raw Milk Samples Collected from Holstein Cows Which Had K. pneumoniae Clinical Mastitis

16S rRNA gene amplicon sequencing was performed on DNA extracted from raw milk samples collected from Holstein cows which experienced a natural K. pneumoniae clinical mastitis infection. The main goal of this project was to understand the milk microbiome of cows before, during, and after, a K. pneumoniae mastitis infection. On select samples, shotgun metagenomic sequencing was performed as well.