Itaconate enhances oncolytic virotherapy via multitarget inhibition of antiviral and inflammatory pathways

4 octyl itaconate (4-OI) is a known anti-inflammatory chemical that activates Nrf2 signaling. Here, we are exploring the capacities of 4-OI to promote the spread of oncolytic Vesicular Stomatitis Virus (VSVd51M) in pLenti control kidney adenocarcinoma 786-O cell line and in Nrf2 knock out 786-O. Overall design: 786-O empty vector (pLenti) control and CRISPR-edited NRF2-KO cells were seeded in 12 well plate and treated with 4-OI for 24 hours (75 uM). Next day, cell were infected with VSVd51M at an MOI of 0.01. At 17 hours post-infection, RNA was extracted using the high pure RNA isolation kit (Roche, 11828665001) and frozen at -70 °C. RNA sequencing was performed in collaboration with BGI Europe Genome Center.