RNA-seq differential expression analysis of Komagataella phaffii CBS2612 under mating conditions

We used RNA sequencing to analyse the differences in gene expression between the homothallic K. phaffii CBS2612 wild type and heterothallic a- and α-type strains during growth in rich medium and under mating conditions. This data was used to investigate mating-type specific gene expression and allowed the identification of the K. phaffii a-factor genes. Examination of expression levels in two different media with wild type and heterothallic a- and α-type cells.