Epigenetic and transcriptomic profiling of granulosa cells and follicular fluid in patients with endometriosis

Endometriosis is the most common disease in the infertility clinic. The compromised oogenesis is the important pathology leading to infertility. Moreover, the impaired oogenesis suffered by patients with endometriosis further compromises the therapeutic outcome. In comparison with the infertile patients with pelvic factors, the patients with endometriosis usually harvest fewer and poorer oocytes during the ovarian stimulation cycle. We aim to take a comprehensive look at the global chromatin accessibility profiles of GCs from the patient with endometriosis, revealing its contribution to the mRNA expression changes in GCs and EVs and ultimately illustrating the cross-talk between GCs and oocytes. Besides that, we intend to discover new molecular markers to predict oocyte development potential. The chromatin accessibility profile of endometriosis GC is significantly altered, thus it could cause the disruption of GC transcriptome and its subsequent function.