Gene expression in human endometrium stromal cells after ovexpression or knockdown of ATF3

In our previous study, we demonstrated that the endometrium of RIF patients display a distinct transcriptional profile and suggests that dysregulation of decidualization may be a cause of implantation failure. Through RNA-seq analysis, we found that ATF3 expression was significantly downregulated in the endometrium of RIF women .In order to explore the role and molecular mechanism of ATF3 in the process of endometrial stromal cell decidualization, we performed RNA-seq to detect the gene profiles in decidualized stromal cells transfected with siATF3, and stromal cells with overexpression of ATF3 by ad-ATF3-His for 48 hours.