nrde-1 is required for silencing nuclear localized RNAs.

Animals of the indicated genotypes were fed bacteria expressing indicated dsRNAs (e.g. lin-15b). eri-1 encodes an exonuclease that is required for the biogenesis of endogenous small interfering RNAs (endo-siRNAs) [36]. eri-1(−) animals have an enhanced RNAi phenotype; they respond more robustly to dsRNA than wild-type animals [27]. Therefore, an eri-1(−) background was used to facilitate phenotypic analysis. The phenotypes (e.g. Multi-vulva) of eri-1(mg366) animals exposed to dsRNA were defined as ‘+’ (∼90–100% of animals with phenotype), the phenotypes of eri-1(mg366);rde-1(ne219) were defined as ‘−’ (0% of animals with phenotype). 50–250 animals were scored blind in each trial (n≥3).