CIL (2012) CIL:39730. CIL. Dataset

TEM images of reconstituted chromatin prepared from 12-mer tandem repeats of a strong nuclear positioning sequence and purified histones. Samples were compacted in the appropriate salt, and mildly crosslinked with formaldehyde to preserve nucleosome-nucleosome interactions, then allowed to decondense in low salt prior to fixation with glutaraldehyde, and shadowing with Pt. (B-D with linker histone and without Mg; J-M) with linker histone and 1 mM MgCl2; (N-S)with linker histone and 4mM MgCl2. The drawings at right show the authors' interpretation of the adjacent micrograph showing crosslinked nucleosomes and the number of nucleosomes included in the 'loop' created by the crosslink.
See Fig 3 in S A Grigoryev et al. 2009 Evidence for heteromorphic chromatin fibers from analysis of nucleosome interactions. Proc Natl Acad Sci USA 106:13317-13322
See Fig 1 in: S A Grigoryev et al. 2009 Evidence for heteromorphic chromatin fibers from analysis of nucleosome interactions. Proc Natl Acad Sci USA 106:13317-13322

本研究展示了由强核定位序列的12碱基串联重复序列和纯化的组蛋白组成的重构染色质的TEM图像。样本在适宜的盐溶液中浓缩，并通过甲醛温和交联以保留核小体-核小体相互作用，随后在低盐溶液中解凝，以固定前用戊二醛处理，并进行铂影处理。（B-D组包含连接组蛋白且无Mg；J-M组包含连接组蛋白和1 mM MgCl2；（N-S组包含连接组蛋白和4mM MgCl2。）右侧的插图展示了作者对相邻显微照片的解释，该照片显示了交联的核小体以及由交联产生的‘环’中包含的核小体数量。参见图3，S.A. Grigoryev等人，2009年，《从核小体相互作用分析中证实异构染色质纤维的存在》，《美国国家科学院院刊》第106卷，第13317-13322页。参见图1，S.A. Grigoryev等人，2009年，《从核小体相互作用分析中证实异构染色质纤维的存在》，《美国国家科学院院刊》第106卷，第13317-13322页。