Effect of depletion of Igf2bp3 on gene expression during VSV infection in RAW 264.7 cells and mice PMs. Effect of depletion of Igf2bp3 on gene expression during VSV infection in RAW 264.7 cells and mice PMs

N6-methyladenosine (m6A) modification is the most abundant RNA modification in both host mRNA and viral RNA transcripts. Dynamic regulation and recognition of m6A modification widely participate in post-transcriptional regulation of many biological processes. This project aims to define the regulation role of IGF2BP3, a m6A recognition protein, in viral infection, especially in RNA viral infection. In this study, the transcriptomes of Vesicular stomatitis virus (VSV)-infected WT/Igf2bp3-KO RAW264.7 cells and mouse peritoneal macrophages (PMs) were analyzed, suggesting the role of IGF2BP3 in the host's innate immune responses during viral infection and enhancement. Overall design: WT/Igf2bp3-KO RAW264.7 cells and PMs were infected VSV-GFP (MOI=1) for 8 hours. Total RNAs from the cells were then extracted using the Trizol reagent, following manufacturer' s instructions. Subsequently, the cDNA libraries were prepared using the VAHTS Universal V8 RNA-seq Library Prep Kit for Illumina (No. NR605, Vazyme) and sequenced on Novaseq 6000 (Illumina).