Library generation information.

aIn CAYC + CAYF there were many different cDNA libraries constructed from seven tissue specific pooled RNA isolates resulting from an array of experiments [19]. These libraries were non-normalized.
bIn CCAG there was one library made from an RNA sample that was pooled with equal quantity of RNA per treatment for each tissue. This library was normalized to increase EST diversity.
cTreatment Conditions Descriptions:
 1. Field collected crabs that were acclimatized across latitudinal (north-south) and seasonal (winter-summer) gradients, heat shock to 30°C, cold shock to 0°C, acclimated to 8°, 12°, 15°, 18°, 22° and 25°C for 2 to 60 days.
 2. Heat 30°C, 4 h (2 h, 4 h, and 6 h 15°C recovery), Cold 2°C, 4 h (2 h, 4 h, and 6 h 15°C recovery), H2O2, 0.5 mM (18 h), CdCl2, 50 µM (24 h), Selenate, 50 µM (24 h), Selenite, 50 µM (24 h), Hypersalinity, 54‰ (18 h), Hyposalinity, 13‰ (18 h), Desiccation (24 h), Hypoxia, 2 h (20 min normoxia recovery), Starvation, 15d (2 h postprandial), Insecticide, 1spray Pyrethrin/200 ml, 5 min (4 h recovery).
 3. Acclimated for 1–7 days in San Francisco Bay water (salinity 25–32‰)
 4. Acclimated for 1 month to 8°, 15°, 18°, & 25°C
 5. Field acclimatized, north-south, winter-summer
 6. Acclimated for 1 month to 7°, 19°C
 7. Acclimated to 1 month in a thermally fluctuating condition (8:18°C, 12 h:12 h)
dEqual amounts of RNA were mixed from each Pooled tissue/treatment to make the pooled RNA sample used to make the CCAG normalized library.