The effect RARG fusion genes or PML-RARA overexpression in human CD34+ cells on the gene expression.

Currently, AML with RARG rearrangements (RARG AML), including NUP98-RARG, PML-RARG, CPSF6-RARG, NPM1-RARG, and HNRNPC-RARG, has been identified as a novel entity of AML. Overexpression of RARG fusions in human CD34+ cells promoted the expansion of immature myeloid cells, reduced the percentage of mature granulocytes, inhibited the differentiation of myeloid cells, and self-renewal abilities of hCD34+ cells. To understand how RARG fusion proteins regulate their target genes in the hematopoietic system, we first expressed HA-tagged X-RARG fusions or HA-tagged PML-RARA in hCD34+ cells. We then analyzed the differential genes expression in hCD34+ cells with or without RARG fusion genes or PML-RARA overexpression through RNA-seq. To analyzed the differential genes expression in hCD34+ cells with or without RARG fusion genes or PML-RARA overexpression, human CD34+ cells infected with control or RARG fusions retrovirus were sorted for GFP positive cells. Then the mRNA profiles of human CD34+ cells with or without RARG fusion genes or PML-RARA overexpression were generated by deep sequencing, two repeats each group, using Illumina HiSeq 6000.