RNA-seq of mice genital ridges from males and females at embryonic days E11 and E12

The aim of the study was to identify differentially expressed genes, isoforms and AS modifications accompanying Gonadal Sex Determination in mice. We performed a Rna-Seq analysis of XX and XY gonads during sex determination on embryonic days 11 (E11) and 12 (E12). RNA-seq libraries were prepared from grouped gonads from E11 and E12 males and females. The cDNA libraries were sequenced using a HiSeq2500 v4 chemistry system本研究旨在鉴定伴随小鼠性腺性别决定（Gonadal Sex Determination）过程的差异表达基因、转录本亚型以及可变剪接（Alternative Splicing，AS）修饰。我们针对胚胎第11天（E11）与第12天（E12）性别决定阶段的XX、XY性腺开展了RNA测序（RNA-Seq）分析。测序文库由E11、E12阶段的雌雄小鼠混合性腺样本制备得到。本次cDNA文库测序采用HiSeq2500 v4化学试剂系统完成。