Expression data from HoxA9wt/Meis1 vs. HoxA9AA/Meis1 mouse models

To verify the impact of eIF4E activation on HoxA9-induced leukemic transformation in vivo, we made a double point mutation (HoxA9AA) to disrupt the physical and functional interaction between eIF4E and HOXA9. Microarray analysis of transformed leukemic cell lines derived from HoxA9wt/Meis1 and HoxA9AA/Meis1 leukemic cells presented highly similar transcriptome profile, without any significant changes of HoxA9 gene signature. we transplanted CD45.2+ LSK cells transduced with Meis1 and either HoxA9wt-GFP or HoxA9AA-GFP transduced into lethally irradiated CD45.1+ recipient mice. Microarrays were done on RNA from BMCs harvested at disease onset. 3 Hoxa9wt/Meis1 and 3Hoxa9AA/Meis1 individuals (biological replicates) were run.