RNA-seq analysis of vehicle and DOPPA-treated human cord blood CD34+ cells

In this study, we did RNA-sequencing analysis to check the transcriptome of vehicle and DOPPA-treated CD34+ cells after 4 days ex-vivo culture. We noticed that expression of many genes which encode cell surface proteins including ITGA6 (CD49f) were significantly up-regulated, FDR-adjusted p-value < 0.01 and the fold change (FC) > 2, by DOPPA treatment. Gene ontology (GO) analysis revealed that many GO functions related to cell surface or plasma membrane related GOs which all include ITGA6 were significantly enriched in up-regulated differentially expressed genes (DEGs) including ITGA6 after DOPPA treatment. These data suggest that DOPPA treatment promotes transcription of ITGA6. Identification of the differentially expressed mRNA profiles in vehicle and DOPPA expanded human cord blood CD34+ cells.