Genome-wide Profiling of Long Noncoding RNA Expression Patterns in Thoracic Aorta from Spontaneously Hypertensive Rats.
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE145854
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We used lncRNA-mRNA micro-array to analyze the transcriptome in the thoracic aorta tissue of 24 wees-old WKY and spontaneously hypertensive rats (SHRs). Tail blood pressure of WKY and SHRs was measured to confirm the hypertension. The thoracic aortae (1.5-2.0cm-long) of WKY and SHRs were quickly removed, cleaned of adhering fat, and connective tissue, then snap-frozen and stored in liquid nitrogen. Total RNA was extracted using Trizol reagent according to the instructions provided by the manufacturer. The Affymetrix GeneChip® Rat Gene 2.0 ST Array was used to analyze the expression profile of lncRNAs and mRNAs in thoracic aortae from adult WKY and SHRs based on the manufacturer’s standard protocols.
创建时间：
2021-01-04
