RNA accumulation in aged endothelial cells links an interferon gene signature to cellular senescence

RNA-seq was performed on early passage and senescent human retinal microvascular endothel cells (HRMECs), to determine transcriptomic changes. Replicative and Etoposide-induced senescent HRMECs senescence-associated gene signature and upregulation of an interferon related gene signature. Overall design: Sequencing of total mRNA from three independent clones, comparing early-passage human retinal microvascular endothelial cells (HRMECs) to replicative and Etoposide-induced senescent counterparts.