Data analysis of the quantitative Crystal toxins lethal concentration assays.

†The range of the LC50 values for Cry5B in N2 animals varies from 8.33 to 20.55 µg/ml, because the absolute quality and measured quantity of toxins varies from different fermentation batches. We performed each experiment using the same batch of the purified toxins.§The absolute LC50 values of these mutant worms can not be calculated by Probit, because the limitation of the solubility of Cry5B and therefore we cannot achieve a 100% killing concentration for these mutants. We used 120 µg/ml, the maximum dose of Cry5B, for the statistical calculations of these resistant mutants.