Investigation of myocardial retionic acid signaling during heart regeneration in zebrafish

Retinoic acid (RA) signaling is required for heart regeneration in zebrafish, but the mechanism of RA-mediated cardiomyocyte proliferation remains unclear. To investigate the downstream mechanism of RA-mediated cardiomyocyte proliferation, we generated a new transgenic line in which a loxP-flanked stop cassette blocks the expression of a dominant-negative form of RA receptor a (b-actin2:LSL-dn-rara), and crossed this strain with a cardiomyocyte-specific inducible Cre driver line (cmlc2:CreER). We performed ventricular resection surgery on cmlc2:CreER; b-actin2:LSL-dn-rara fish with and without RA-inhibition and collected 3 and 7 days post-injury hearts to compare the gene expression profile of regenerating hearts. This project contains raw sequencing data sets of these hearts that were used to identify genes of which expression is regulated by RA-signaling during regeneration.