Associative appetitive olfactory conditioning with Sucrose or Sorbose in Drosophila at 4 hours post training.

To obtain a comprehensive view of genes contributing to long-term memory we performed mRNA sequencing from single Drosophila heads following behavioral training that produces long-lasting memory. Drosophila were starved and trained with an appetitive conditioning paradigm involving Sucrose or Sorbose, and then collected prior to or 4 hours post-training, 4 to 6 replicates each, for RNA-Seq analysis of the fly heads with an Illumina HiSeq 2000.