Single cell RNAseq of adoptively transferred Tregs in rhesus macaques
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE139200
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Animal R.401 was infused with ex-vivo expanded T-regulatory cells. After adoptive transfer, PBMC were obtained and stained with surface antibodies. Single cell libraries from sorted populations were generated using a UMI-based droplet-partitioning platform (10X Genomics) and sequenced using a NextSeq 500 (Illumina). Resulting reads were processed using Cellranger software (10X Genomics) and downstream analysis was performed using Monocle6,7 using a negative binomial model of distribution with fixed variance. Time-course
创建时间:
2019-10-24



