First Metagenome-Assembled Genomes from the Historic Morrow Plots

This manuscript presents the first genome resolved metagenomic resource generated from the historic Morrow Plots, one of the longest running agricultural field experiments in the world. Using 33 shotgun metagenomes collected from soils under different crop rotation and fertilization treatments, the study reconstructed 230 metagenome assembled genomes, including both archaeal and bacterial lineages. The dataset highlights management associated patterns in soil microbial community structure, with a particular emphasis on archaeal ammonia oxidizers and other nitrogen cycling microorganisms. In addition to genome reconstruction, the study provides taxonomic assignments, genome quality statistics, and treatment resolved abundance profiles, creating a valuable reference for investigating how long term agricultural practices influence soil microbial ecology, nitrogen cycling, and soil health.