High-Spatiotemporal-Resolution Transcriptomes Insights into Fruit Development and Ripening in Citrus sinensis

To excavate the underlying molecular regulation network that during citrus fruit development and ripening, we used RNA-seq to generate high-resolution profiles of global gene expression in four different fruit tissues at six development stages. Using weighted gene coexpression network analysis, we identified modules of coexpressed genes and hub genes of tissue-specific networks. In general, this study was aimed to uncover the new molecular insights into citrus fruit development and ripening, and to reveal the specific nonclimacteric characteristics of citrus fruit. Overall design: Fruit samples of the 'Fengjie 72-1' navel orange (C. sinensis L. Osbeck) were harvested at 50, 80, 120, 150, 180 and 220 days after flowring (DAF). Three trees as one biological replicate. Three biological replicates (three trees per replicate) were harvested at each developmental stage. Twelve representative fruits were sampled from each tree at each developmental stage.The four fruit tissues (epicarp, albedo, segment membrane and juice sac) were isolated by hand dissection. A totlal of 72 transcroptomes were sequeced.