Sequences of miRNAs and probes used in microarray and qRT-PCR.
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Free energy for the probe-target binding was calculated using the thermodynamic parameters for RNA/DNA hybrid duplexes from Sugimoto et al. [69]. Tm was calculated according to the formula reported in Wei et al. [70] using the thermodynamic parameters for RNA/DNA hybrid duplexes from Sugimoto et al. [69] that consisted of the following parameters: [Na+] = 1 M, Ct (oligonucleotide concentration) = 100 uM, A (helix initiation factor) = −10.8 cal/KM, F (correction term per1% Formamide) = 0.63°C, Formamide concentration = 35%, R (universal gas constant) = 1.987 cal/KM.
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2015-12-02
