Targeting BRD4 Ameliorates Experimental Emphysema Via Suppressing both M1 and M2 alveolar macrophage polarization-3 [CUT&TAG]

BRD4 inhibition suppressed M2 macrophage polarization. Particularly, we deeply investigated the underlying molecular mechanism of how BRD4 epigenetically regulate M2 genes expression. And finished the cut&tag experiment using BRD4, H3K27ac, IRF4 as antibody to find out the binding sites change in M2 macrophage treated with ARV825. To investigate BRD4 degraders effect on the binding sites change of BRD4, H3K27ac, IRF4 in IL-4 induced M2 aveolar macrophage , we finished the RNA-seq experiment.