Quantification of dengue virus RNA levels and normalized RNA expression ratios in dengue virus-infected cells.

CT values represent the mean ± standard deviation (SD) of three independent experiments, performed in triplicate. CT values were analyzed using the Relative Expression Software Tool (REST), wherein expression ratios are generated and tested for significance by a randomization test [26].aNormalized dengue RNA expression represents the ratio of dengue RNA levels in NF90 knockdown cells relative to dengue RNA levels in control cells, each normalized to actin RNA levels.