Differential transcriptomics analysis of IPEC-J2 cells single or co-infected with Porcine epidemic diarrhea virus and transmissible gastroenteritis virus

In this study, intestinal epithelial cells IPEC-J2 were single or co-infected with PEDV and/or TGEV, followed by the comparison of differential expressed genes, especially ISGs, between different groups via transcriptomics analysis and real-time qPCR. The results showed that differentially expressed genes (DEGs) can be detected in the cells infected with PEDV, TGEV, and PEDV+TGEV at 12, 24, and 48 hpi, and the number of DEGs was the highest at 24 hpi. The DEGs mainly annotated to the GO terms of protein binding, immune system process, organelle part, intracellular organelle part. Furthermore, 90 ISGs were up-regulated during PEDV or TGEV infection, which were subcellular located in the nucleus and cytosol, including IRF1, IRF7, IFITM1, and IFITM3.