Cell-autonomous effects of APOE4 in restricting microglial response in brain homeostasis and Alzheimer's disease

Cells in the cortex of mice expressing apoE3 or apoE4 in the microglia were isolated and subjected to the single-cell RNA seq to investigate the effects of microglia/CNS-associated macrophages (CAMs) apoE on the brain transcriptomic profiles. Our data revealed that microglia/CAM-expressed apoE3 promotes antigen presentation and immune patnways, whereas apoE4 down-regulates complement and promotes stress-related responses. Overall design: Amyloid model mice with or without expressing apoE3 or apoE4 in microglia/CAMs (total 24 mice) were analyzed. N=6/group (3 male and female mice per group). The four groups are Ctrl (C) vs. Tamoxifen (T)-induced apoE3 or apoE4.