Kinetic parameters of PcGSTFuAs and CcGSTFuAs in enzymatic assays.
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The apparent Km values for all compounds were determined using a concentration range of 0.1–10 mM in the presence of 5 mM GSH. The Km value for GSH was determined with 1 mM Phenethyl-ITC for PcGSTFuA1, PcGSTFuA3, CcGSTFuA2461, CcGSTFuA6801 and 50 µM CMFDA for CcGSTFuA6800 with a concentration range of 0.01 to 10 mM GSH. The apparent Km and kcat values were calculated by nonlinear regression using the Michaelis-Menten equation (r2>0.99). Data are represented as mean ± S.D. (n±3). ND: Not Detected. The detection limit was estimated at 0.5 mUI.
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2015-12-02
