Results of qPCR quantification of P. murina mtLSU RNA gene in the lungs of the nine distinct mice groups enrolled in the study.

Note: The level of infection of the samples was estimated using the standard curve determined in the study: Cq = −5.3661 log10 (mtLSU rRNA concentration) +68.7078; R2 = 0.9933; Quantification cycles (Cq) were obtained in duplicate and the average Cq were calculated. The maximum Cq was 42.82 (dilution 1∶300 of the initial P. murina 2×107 nuclei/ml suspension). Pm- Pneumocystis murina;aThe control mice which had not been infected by P. murina, did not presente Pneumocystis DNA in the lungs, indicating that the animals used in the study were free of P. murina prior to exposure to the microorganism. The Group 1 qPCR values is an average of one mouse dosed in duplicate for days 0 and day 14, respectively and two mice dosed each in duplicate for day 21; b The same fungal burden at day 0 resulted from the average of four mice each dosed in duplicate; c the qPCR values is an average of one mouse dosed in duplicate for day 14, and three mice dosed each in duplicate for day 21; MB – below the minimal fungal burden detectable by qPCR; UD - reduction to undectable levels.