A survey of transcriptome in Bradysia odoriphaga using PacBio single-molecule long-read sequencing

PacBio platform were used to sequencing transcriptome of Bradysia odoriphaga and produce a single-molecule long-read dataset. The isoforms after polish and removed redundancy were then annotated by BLASTX using public databases. Isoforms function were predicted by categorized the isoforms into Gene Ontology (GO), Cluster of Orthologous Groups (COG), and grouped into pathways using the Kyoto Encyclopedia of Genes and Genomes (KEGG). SSRs in isoforms were identified and the isoforms related to insecticide resistance and metabolisms were analysis.