Identification of downstream target genes of transcription factor ZEB1, lncRNA FGD5-AS1 and miR-34a-5p in gastric cancer

As one of the most abundant lncRNAs in cells, the biological function of lncRNA FGD5-AS1 remains largely unclear in cancers, especially in gastric cancer (GC). In addition, the reason for such high levels of FGD5-AS1 in cells remains unknown. In this study, FGD5-AS1 was proved to be a ZEB1-related lncRNA which was overexpressed and predicted poor prognosis in GC. Knockdown of FGD5-AS1 decreased GC proliferation in vitro and in vivo. To reveal the molecular mechanism by which FGD5-AS1 drives gastric cancer progression, we established GC cell lines knocked down the expression of FGD5-AS1. The transcriptome sequencing studies were performed by deep sequencing. Exploration of downstream target genes of transcription factor ZEB1 in gastric cancer cell lines. The GC cell lines were transfected with miR-34a-5p mimics and negative control siRNAs. After effective transcrection, the genes downregulated by miR-34a-5p were screened by RNA sequencing.