SLC33A1 exports oxidized glutathione to maintain endoplasmic reticulum redox homeostasis [KPK_Slc33a1_KO_TUNC]

Endoplasmic reticulum stress and redox homeostasis influence protein folding and unfolded protein response signaling. To examine transcriptional responses associated with Slc33a1 loss and complementation, we performed RNA sequencing of KPK cells with Slc33a1 knockout and cells complemented with either empty vector or cDNA constructs. Cells were treated with either DMSO or tunicamycin to induce ER stress, and total RNA was collected for sequencing. This dataset provides transcriptome profiles that enable comparison of gene expression changes across genotypes and treatment conditions. Overall design: KPK cells with Slc33a1 knockout and complemented lines were cultured and treated with either DMSO or tunicamycin. Total RNA was extracted and libraries were prepared for RNA sequencing. Biological replicates were generated for each condition to compare transcriptional responses to ER stress in different genetic backgrounds.