plankton diversity of the East China Sea

For investigating whether the larvae of southern intertidal species can be found in the north of Yangtze River mouth, plankton samples were collected on August 2014 and June 2015 around the Yangtze River Mouth and Yellow Sea coast (Supporting Information Figure S1) using a plankton net (Mesh size 0.155 μm) and were stored at -20°C for metabarcoding analysis. A COI gene fragment from total genomic DNA was pyrosequenced on the Roche 454 GS FLX+ platform at Xiamen University, China.