Artificial Melanogenesis by Confining Melanin/Polydopamine Production Inside Polymersomes

Data underlying the figures in the publication “Artificial melanogenesis by confining melanin/polydopamine production inside polymersomes”, published in Macromolecular Bioscience, 2021.

Table of contents:

1. Figure 1; Zip. archive containing the experimental data for Figure 1. TEM micrographs and corresponding DLS and SLS revealing the morphology and size of (A) Melanin-polymersomes (with Tyrosinase), (B) PDA-polymersomes (with Tyrosinase), (C) empty polymersomes rehydrated with PBS only, and (D) Tyrosinase-polymersomes.

2. Figure 2; Zip. archive containing the experimental data for Figure 2. (A) Zeta potential values showing the absence of Dopamine, L-DOPA or Tyrosinase adsorption at the surface of polymersomes. TEM micrographs of polymersome clusters formed after adding (B) L-DOPA with Tyrosinase, (C) Dopamine with Tyrosinase, (D) L-DOPA and (E) Dopamine to empty polymersomes.

3. Figure 3; Zip. archive containing the experimental data of the graphs in Figure 3. Melanin and PDA formation within polymersomes monitored via UV-vis spectroscopy. Absorption spectra measured at t = 0 (grey), t=12 h (cyan) and t=24 h (orange) of incubation at 37 °C of (A) Empty polymersomes, (B) Tyrosinase polymersomes, (C) L-DOPA polymersomes, (D) L-DOPA polymersomes (with Tyrosinase), (E) Dopamine polymersomes, (F) Dopamine polymersomes (with Tyrosinase).

4. Figure 4; Zip. archive containing the experimental data for Figure 4. Interaction of polymersome-based melanosome mimics with HaCaT cells. (A) Cell proliferation assay showing the non-toxicity of different polymersomes encapsulating dopamine and Tyrosinase (dashed purple), L-DOPA and Tyrosinase (dashed grey), dopamine (purple), L-DOPA (grey), PBS (yellow), Tyrosinase (dashed yellow) compared to control PBS without polymersomes (blue). Cells were incubated with respective polymersomes for 24 h. (B) Cell proliferation assay showing the cytotoxicity of free L-DOPA/dopamine (with and without Tyrosinase) compared to PBS. (C) Cell proliferation assay showing the cytotoxicity/cell protection effect of different kinds of polymersomes after 40 min of UV-irradiation of cells treated for 24 h with polymersomes. (D) CLSM images showing perinuclear localization of model polymersomes (encapsulating fluorescent Atto-488) in keratinocytes. Statistical significance was shown as p values < 0.02.