RNA-seq investigating mouse derived and iPSC derived macrophage populations before and after pulmonary transplantation

The key aim of this experiment is to characterize the iPSC-Mf population before and after pulmonary transplantation. For this purpose the following cell populations were compared: (i) transplanted iPSC-Mf, (ii) Mf obtained by in vitro differentiation of murine lineage-negative bone marrow cells (BM-Mf), (iii) non-differentiated CD45.1 iPSC, (iv) murine alveolar Mf (AMf) isolated from the BALF of healthy control mice, and (v) iPSC-Mf recovered from the transplanted animals two months after (PMT-Mf).