Differentially expressed genes between participants with high versus low myosin heavy chain fragmentation 24 hours following exercise

To gain further insight into the relevance of post-exercise skeletal muscle myosin heavy chain fragmentation (MyHCfrag), we performed a low versus high MyHCfrag analysis whereby participants were clustered into tertiles based on 24h post-exercise MyHCfrag values. In the highest tertile (n=6, 5 males, 2 TRAD), MyHCfrag was elevated at 24h compared to Pre (p<0.001) and compared to 24h in the lowest tertile (p<0.001). Conversely, in the lowest tertile (n=6, 2 males, 5 TRAD), MyHCfrag was unaffected at 24h vs Pre (p>0.05). These top and bottom tertiles were examined for differential gene expression patterns. These data summarize the 24-hour post-exercise DEGs between tertiles. Relative to low MyHCfrag participants, 364 transcripts were lower and 435 transcripts were higher in high MyHCfrag participants (>1.5-fold change, q < 0.10).