File S1 - Lipid Raft Is Required for PSGL-1 Ligation Induced HL-60 Cell Adhesion on ICAM-1

Figure A, MβCD treatment does not affect the expression of PSGL-1 and β2 integrin. HL-60 cells were incubated with or without MβCD (10 mM), then the cells were stained with KPL-1(left), IB4(right), followed by FITC-labeled anti-human IgG, and analyzed by flow cytometry. Results are representative of three independent experiments. Figure B, Piceatannol treatment does not affect the expression of β2 integrin. HL-60 cells were incubated with or without Piceatannol (10 µM) and stained with IB4 or human IgG followed by FITC-labeled anti-human IgG, and analyzed by flow cytometry. Results are representative of three independent experiments. (DOC)