Comparison of estimates of Ne.

TS stands for ‘this study’. RE stands for restriction enzyme. The experimental setups were classified according to which part of the transmission or infection process was considered for Ne was estimated: I. Full transmission process: systemic leaf – vector – inoculated leaf – systemic leaf. II. Partial infection process: inoculum - inoculated leaf. III. Partial infection process: inoculated leaf – systemic leaf. Doses are given as the number of RNA molecules measured in purified virions stocks for data from this study, or as ng of purified virions per inoculated plant for study [30]. Ne estimates are given with the standard error of the mean, or the reported range of estimates. Estimates of Ne are made for (systemically) infected hosts, with the exception of those estimates marked with an asterisk (*) which are estimates made over the whole host population, not only the infected hosts.aOnly data from the wild-type/P20L experiment presented, as other experiments with other genotype combinations gave highly similar results.bNe estimates made for different mixtures of an infective and non-infective genotype used, leading to different levels of infection. The mean level of infection is given.cThe estimated number of virions transmitted per aphid; 2 aphids per plant were used in the study.dThe mean number of genotypes found in infected plants is given, which is a rough approximation of Ne as the number of genotypes used (12) is much higher than the number of genotypes found in infected plants.eF1F2F3 and F1L2F3 genotypes.fF1F2F3 and F1L2F3 genotypes, and satellite.