Gene expression data for three mouse auditory brainstem nuclei at two times of development

Genome-wide gene expression was obtained in three auditory brainstem nuclei (defined below), at two different ages in mice, postnatal day (P)3 and P14. The primary aim was to identify genes which are differentially expressed between the medial nucleus of the trapezoid body (MNTB) and the superior olive (LSO), at both age groups. Tissue samples from the ventral cochlear nucleus (VCN), the medial nucleus of the trapezoid body (MNTB) and the lateral superior olive (LSO) were collected from 200 ÎŒm thick fresh transverse mouse brainstem slices prepared with a vibratome tissue slicer. Individual brainstem nuclei were dissected out with fine forceps under optical control with a binocular. For each nucleus (VCN, MNTB, and LSO), the tissue from six male C57Bl6 mice at postnatal day (P) 3, and from three male C57Bl6 mice at P14 was pooled. The RNA was isolated with the RNeasy Micro kit (Qiagen); RNA samples were amplified using a WT ovation Pico RNA amplification (Nugen, CA, USA). The cDNA was hybridized onto mouse Affymetrix microarrays 430 2.0 (Affymetrix, Santa Clara, USA).本数据集获取了小鼠3个听觉脑干核团（auditory brainstem nuclei，定义见下文）在两个不同生后日龄——生后第3天（P3）与生后第14天（P14）——的全基因组基因表达（Genome-wide gene expression）谱。本研究的核心目标为：在两个日龄组中分别鉴定斜方体内侧核（medial nucleus of the trapezoid body, MNTB）与外侧上橄榄核（lateral superior olive, LSO）之间存在差异表达的基因。研究人员使用振动切片机（vibratome tissue slicer）制备厚度为200 μm的新鲜小鼠脑干横向切片，从中采集腹侧耳蜗核（ventral cochlear nucleus, VCN）、斜方体内侧核（MNTB）及外侧上橄榄核（LSO）的组织样本；在双目显微镜（binocular）的光学操控下，使用精细镊分离得到单个脑干核团。针对每个核团（VCN、MNTB、LSO），分别混合6只生后第3天的雄性C57Bl6小鼠的组织样本，以及3只生后第14天的雄性C57Bl6小鼠的组织样本。使用RNeasy Micro试剂盒（RNeasy Micro kit, Qiagen）提取总RNA；采用WT Ovation Pico RNA扩增试剂盒（WT ovation Pico RNA amplification, Nugen, CA, USA）对RNA样本进行扩增。最终将合成的cDNA杂交至小鼠Affymetrix 430 2.0基因芯片（mouse Affymetrix microarrays 430 2.0, Affymetrix, Santa Clara, USA）。