Substrate specificity of Ct43Araf and CtGH43 from C. thermocellum.
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All the assays were performed at 50°C using 20 mM sodium phosphate (pH 5.7) buffer for aCt43Araf and 20 mM sodium acetate (pH 5.4) buffer for bCtGH43. The assays were performed in triplicates. The incubation time and other conditions for reducing sugar estimation were as same as described in the Materials and Methods section.*/**It was prepared by Megazyme (Ireland) via controlled enzymatic hydrolysis of de branched sugar beet arabinan as described in the manufacturer’s instructions.ND = No activity detected.
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2015-12-02



