Data underlying the publication: Trigger factor accelerates nascent chain compaction and folding

This dataset contains data collected during correlated single-molecule force spectroscopy and multi-color confocal laser scanning spectroscopy experiments at AMOLF, as part of Katharina Till's PhD Thesis project. The objective of the project behind this data was to study the conformational control mechanisms of chaperone assisted protein folding. Using selective ribosome profiling and optical tweezers with correlated single-molecule fluorescence, with dihydrofolate reductase (DHFR) as a model system, we show that the <em>Escherichia coli</em> chaperone trigger factor (TF) accelerates nascent chain folding. The data included in this data set has been organised per figure of the corresponding to be published publication. The excel sheet contains several tabs containing the underlying data to the figures they are named after. The data is being made public both to act as supplementary data for publications and the PhD thesis of Katharina Till and in order for other researchers to use this data in their own work.<br>

本数据集包含了作为Katharina Till博士论文项目一部分、在AMOLF开展的关联单分子力谱（correlated single-molecule force spectroscopy）与多色共聚焦激光扫描光谱（multi-color confocal laser scanning spectroscopy）实验中采集的实验数据。本数据集依托的研究项目旨在探究分子伴侣辅助蛋白质折叠的构象调控机制。本研究以二氢叶酸还原酶（dihydrofolate reductase，DHFR）为模型系统，结合选择性核糖体谱分析与搭载关联单分子荧光检测的光镊技术，证实大肠杆菌（Escherichia coli）来源的分子伴侣触发因子（trigger factor，TF）可加速新生肽链的折叠过程。本数据集内的数据已按照对应待发表论文中的各幅图表进行分类整理。该Excel文件包含多个工作表，各工作表以其所对应图表的名称命名，存储了支撑各图表的原始实验数据。本次公开本数据集，既可为Katharina Till的博士论文及相关发表论文提供补充数据，也可供其他科研人员在其研究工作中使用本数据集。