Data from: The induction of the fibroblast extracellular senescence metabolome is a dynamic process

Cellular senescence is often associated with irreparable DNA double strand
breaks (IrrDSBs) which accumulate with chronological age (IrrDSBsen). The
removal of senescent cells ameliorates several age-related diseases in
mice but the translation of these findings into a clinical setting would
be aided by the characterisation of non-invasive biomarkers of senescent
cells. Several serum metabolites are independent indicators of
chronological age and some of these accumulate outside senescent
fibroblasts independently of cell cycle arrest, repairable DNA breaks and
cell size (the extracellular senescence metabolome, or ESM). The
post-mitotic phase of senescence is dynamic, making the detection of
senescent cells in vivo difficult. An unbiased metabolomic screen of the
IrrDSBsen fibroblast ESM also showed differences in the times of
initiation and maintenance of different metabolites but generally the ESM
altered progressively over the 20 day study period unlike the reported
transcriptional profiles. This more detailed analysis of IrrDSBsen
identified several new ESM metabolites that are associated with
chronological ageing. Targeted analysis of citrate confirmed the dynamic
nature of this metabolite in two cell lines and revealed its independence
from the senescence effector p16INK4A. These data will aid our
understanding of metabolic signatures of ageing and their relationship to
cellular senescence and IrrDSBs.