Surface plasmon resonance dataset of Trypanosoma evansi RoTat1.2 variant surface glycoprotein antigen interaction with monoclonal antibody- coated biosensors

The surface plasmon resonance (SPR) bio-(immuno-)sensors are being developed for the diagnosis of infectious diseases, cancers, food safety, etc. The SPR immunosensor using a monoclonal antibody as the capture biomolecule coated onto the gold chip allows direct, rapid, real-time, label-free, quantitative and cost-effective detection of the target antigens as analyte in a test sample. We developed for the first time SPR immunosensors using two monoclonal antibodies, viz., 2E11 (IgG1) and 1C2 (IgG1), produced in our laboratory for real-time, label-free, and rapid detection of their target antigen, i.e., Trypanosoma evansi RoTat 1.2 variant surface glycoprotein (VSG) in sera samples from the laboratory rodents and the field bovines [File 1 pdf].
First, we produced by the hybridoma technique several mAbs that reacted with the T. evansi RoTat 1.2 lysate Ags [File 2 pdf]. One of these mAbs, viz., 2E11 mAb was then used to immunoprecipitate the target Ag in the parasite lysate [Fig. 1A & 1B; File 3 pdf], which was then identified as T. evansi VSG by mass spectrometry [Fig. 2; File 3 pdf & File 4 Excel data]. Both 2E11 and 1C2 mAbs reacted with the VSG Ag in the Western blots [Fig. 3; File 3 pdf]. Then, the interactions of these mAbs with the above VSG Ag in the parasite lysate were analyzed by the respective SPR-immunosensor. The immunosensor was developed by binding of the biotinylated mAbs onto streptavidin immobilized on the gold chip [Dutra, RF and Kubota, LT. (2006). Clinica Chimica Acta. 379, 114-120]. The equilibrium dissociation constants (KD= kd/ka) of mAbs-VSG were determined to be 127 nM (ka=196.4 ± 61.9 s-M-; kd=2.51E-05 s-) for 2E11 mAb and 290 pM (ka=4616.1 ± 170.1 s-M-; kd=1.36E-06 s-) for 1C2 mAb (Table 1 pdf; Files 5 & 6 Excel data; Fig. 4-5 pdf).
Further, we produced the SPR data and the sensograms of the interactions of 2E11 and 1C2 mAbs with the VSG Ag in the sera samples of the parasite-infected laboratory rodents as well as the test sera samples from the field cattle and buffaloes [File 7 with Fig. 6-11 Excel data; Fig. 6-11 pdf; File 8 with Fig. 13-17 Excel data; Fig. 12-17 pdf]. In addition, the kinetic parameters of the mAb interactions with two synthetic peptide mimotopes of the VSG were determined (Table 1 pdf; File 9 with Fig. 18-21 Excel data; Fig. 18-21 pdf). The peptide mimotopes of VSG were previously selected by PhD-12 phage display library panning against 2E11 and 1C2 mAbs in another study [Dataset reference: Mendeley Data, V2, doi: 10.17632/bs6pbskc8n.2]. These data provide valuable information for developing the real-time, label-free, SPR- based immunosensors for the diagnosis of surra caused by Trypanosoma evansi infection in a wide variety of domestic, zoo, and wildlife animal species.