circRNA and mRNA transcriptome of Human Daoy and HEPM cells in the context of Hedgehog signaling activation

Circular RNAs have largely emerged as novel regulators of human biology, including brain function and cancer development. On the other hand, the Hedgehog pathway has established roles in regulating biological processes, including tumorigenesis. Here, the circular RNA transcriptome, in the context of Hedgehog signaling activation of medulloblastoma Daoy and human embryonic palatal mesenchyme HEPM cells, was determined. We have identified the circRNA transcriptome of two human cell lines, the SHH medulloblastoma Daoy and the embryonic palatal mesenchyme HEPM cells, in the context of Hedgehog signaling activation with the purified ligand SHH or the small molecule SAG. Total RNA from treated and untreated cells was divided into two fractions to enrich for either linear mRNAs (poly(A) selection) or circRNAs (ribosomal RNA depletion and RNase R treatment) and subjected to Illumina RNA-seq.