Quantification of the phase shifts in <i>lak</i> versus double mutant larvae kept in DD or submitted to pulses of white light at CT16 or CT21.

As for Tables 1 and 2, the Δphase is the difference between the phase of the two last cycles and the phase of the two first cycles. A Phase shift is observed in DD owing to the period that is close to 25 hours which generates a ~1 hour-shift every cycle. Phases were calculated with the FFT-NLLS method (biodare2.ed.ac.uk). Upon a pulse of light at CT16 or CT21 a statistical difference is observed between DD and pulsed lak larvae (****. p<0.0001 using a Mann-Whitney two-tailed test). In both phase delays (PD, pulse of light at CT16) and phase advance paradigms (PA. pulse of light at CT21), no statistical difference between lak and double larvae is observed using a Mann-Whitney two-tailed test. For each type of paradigm DD, PD and PA three independent experiments were pooled.