Glucose supply and glycolysis inhibition shape the clinical fate of preterm newborns infected with Staphylococcus epidermidis [whole blood]

To investigate if appropriately adjusted parenteral glucose supply and/or temporary glycolysis inhibition by administration may help to prevent sepsis or improve sepsis outcomes of preterm newborns, we using a preterm pig model inoculated with Staphylococcus epidermidis to mimic the inflammation condition of preterm newborns at birth and treated them with different parenteral nutrition glucose supplyment and glycolysis inhibitor administration. We then performed gene expression profiling analysis using data obtained from RNA-seq of preterm pigs` whole blood, which collected at 12 hours after SE infection, treated with different parenteral glucose supply and glycolysis inhibitor administration. Overall design: Comparative gene expression profiling analysis of RNA-seq data for whole blood samples from preterm pigs with/without S. epidermidis (5×10^5 CFU/ml) infection and DCA administration, pigs received either high or standard parenteral glucose supply.